The purpose of this position is to perform laboratory technical work to assess immune responses under the direction of Lab Management. Technical work will include processing biological specimens, perfoming laboratory assays, collecting and analyzing data, assisting in the development and maintenance of laboratory quality control (QC) methods and records, and reporting on experimental results. The Research Technologist may also work with the clinical or laboratory staff from regional HVTN Clinical Trials Units and with HVTN sites to coordinate specimen and data transfer, data analysis and assay development.
- Isolate, culture, and either cryopreserve or assay leukocyte populations from blood, tissues and mucosal specimens using sterile technique.
- Evaluate immune responses using techniques such as: multiparameter flow cytometry (intracellular cytokine staining, cellular phenotyping), multiplex protein detection, molecular techniques (e.g. DNA/RNA extractions, PCR techniques and next generation sequencing), ELISpot, ELISA, and microscopy.
- Conduct all work according to Good Clinical Laboratory Practice (GCLP) guidelines and auditable-standards, ensuring accurate and timely reporting and record keeping.
- Follow, update, modify and write lab protocols and standard operating procedures (SOPs).
- Order and maintain supplies and prepare and maintain reagents, stocks and cultures necessary to perform the work. Maintain sample and reagent storage information in computerized laboratory information systems.
- Directly execute experiment plans and analyses, which may include but is not limited to: creation of an experiment plan, training of other laboratory staff, completion of laboratory assays, analysis of data, figure creation, and drafting of reports with data interpretation to present to lab management.
- Supervise interns or other lab personnel as assigned by ensuring adequate training, overseeing assays and providing guidance on analysis and troubleshooting, and any other associated tasks to ensure their success.
- Independently develop new assays by conducting literature reviews, communicating with collaborators, designing experiments, optimizing, troubleshooting, analysing and reporting results.
- Transfer newly-developed assays and technologies to other parts of the lab or to collaborators and train lab members to be proficient in those assays.
- Maintain records for experiments and quality control (QC) for the laboratory.
- Operate and carry out basic maintenance and QC of laboratory equipment.
- Share on-call duties with lab personnel to respond to equipment alarms in a timely manner after normal work hours (off-hours).
- Attend and participate in appropriate laboratory meetings.
- Contribute to the development of the laboratory environment (e.g. Laboratory safety).
- Package, ship, receive and store biological specimens in accordance with SOPs and applicable regulations e.g IATA.
- Assume responsibility for certain assigned projects by planning and executing all steps needed for project completion.