Research Technician I/II - Molecular Profiling Team (McElrath Lab)

Job ID
23423
Type
Regular Full-Time
Location
US-WA-Seattle
Category
Laboratory Research Sciences

Overview

Fred Hutchinson Cancer Center is an independent, nonprofit, unified adult cancer care and research center that is clinically integrated with UW Medicine, a world leader in clinical care, research and learning. The first National Cancer Institute-designated cancer center in the Pacific Northwest, Fred Hutch’s global leadership in bone marrow transplantation, HIV/AIDS prevention, immunotherapy, and COVID-19 vaccines has confirmed our reputation as one of the world’s leading cancer, infectious disease and biomedical research centers. Based in Seattle, Fred Hutch operates eight clinical care sites that provide medical oncology, infusion, radiation, proton therapy, and related services, and network affiliations with hospitals in five states. Together, our fully integrated research and clinical care teams seek to discover new cures to the world’s deadliest diseases and make life beyond cancer a reality.

 

At Fred Hutch, we believe that the innovation, collaboration, and rigor that result from diversity and inclusion are critical to our mission of eliminating cancer and related diseases. We seek employees who bring different and innovative ways of seeing the world and solving problems. Fred Hutch is in pursuit of becoming an antiracist organization. We are committed to ensuring that all candidates hired share our commitment to diversity, antiracism, and inclusion.

 

This research opportunity is with the McElrath Lab within the Vaccine & Infectious Disease Division. The Research Technician I/II will work under the direction of the Molecular profiling team manager and other higher level laboratory personnel in support of the antigen specific B cell platform to help with the characterization of antigen specific B cell receptor repertoires in the context of HIV vaccine trials. In particular, they will assist in developing and implementing high-throughput B cell receptor sequencing assays, including BCR PCR, Illumina NGS library preparation, CITE-seq, micro neutralization screening, PCR cloning and transient transfection for monoclonal antibody production and functional screening of these monoclonals. The incumbent will also be responsible for performing a variety of molecular and cellular biology techniques including multiplex PCR, single-cell RT-PCR, digital droplet PCR, maintenance of feeder cells under aseptic conditions, and RNA/DNA isolation. They will be involved in data management, interpretation and reporting on experimental results and assist with data presentation for lab meetings, conferences and manuscripts. The incumbent should be capable of maintaining detailed laboratory records, analyzing data, and reporting experimental results. They will interact closely with other laboratory staff to coordinate both up and down stream processing of samples and needs to a be a good team player. They will assist in the development and maintenance of laboratory quality control (QC) methods and records, which may require adherence to GCLP (Good Clinical Lab Practice) standards.

Responsibilities

  • Perform molecular biology techniques: isolation of RNA/DNA from human lymphocytes for downstream analysis, gene expression by high density multiplex PCR, RT-qPCR, RNA-Seq, RT-PCR amplification for targeted amplicon sequencing of B cell receptor genes (paired and unpaired), including indexing and library preparation for NGS Illumina platform, antibody cloning and purification, monoclonal antibody production by transient transfection.
  • Perform tissue culture techniques: maintenance of cell lines including frozen inventories, transduction of feeder cells with retroviral and lentiviral vectors, virus neutralizations, cytotoxicity assays, in vitro cell manipulations, including peptide stimulations and co-cultures.
  • Perform immunochemical assays such as ELISA, BLI and Western blotting.
  • Operate and carry out basic maintenance and QC of laboratory equipment.
  • Perform and/or optimize new assays and specimen processing methods as needed.
  • May prepare stocks of HIV pseudoviruses for downstream neutralization.
  • Prepare and maintain reagents, stocks, and cultures necessary to perform the work.
  • Independently collect and analyze data. Assist in preparation of data for oral and written presentations.
  • Other duties as directed by supervisor, including conducting literature reviews to assist in determining most suitable methods to be used in research

Qualifications

  • Research Technician I: Bachelor's degree in a related field required. One year of independent research experience in addition to school lab experience is preferred.
  • Research Technician II: Bachelor's degree in a related field and at least two years of work experience in a research laboratory. 
  • Preference given to laboratories with a focus on PCR, NGS library preparation experience and tissue culture techniques. 
  • Experience with Molecular biology techniques such as PCR and NGS library preparation, bacterial cloning as well as aseptic tissue culture techniques is preferred.
  • Great attention to details, well-organized, self-motivated, and able to efficiently manage time and experiments.
  • To be successful in this position, the technician must have scientific curiosity, a high level of self-motivation, ability to take instruction and follow directions, good organizational skills, ability to work and think independently, but also be a team player.
  • Show initiative and be willing to take up new laboratory skills, and communicate well with other members of the research team. Strong skills in data collection and laboratory record keeping are mandatory. Computer skills and experience with statistical analysis and data presentation are desirable.
  • Applicants must be willing to work with biohazardous/infectious agents (e.g., bloodborne pathogens, including HIV-1) under carefully controlled conditions and have the flexibility to work some evenings and weekends.

 

Fred Hutch has a mandatory COVID-19 vaccine requirement, with exceptions only for approved medical or religious accommodations.
As a condition of employment, newly hired employees must provide proof of vaccination or initiate the accommodations process before their first day of employment.

 

A statement describing your commitment and contributions toward greater diversity, equity, inclusion, and antiracism in your career or that will be made through your work at Fred Hutch is requested of all finalists.

Our Commitment to Diversity

We are proud to be an Equal Employment Opportunity (EEO) and Vietnam Era Veterans Readjustment Assistance Act (VEVRAA) Employer. We are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability (physical or mental), marital or veteran status, genetic information, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to our Employee Services Center at hrops@fredhutch.org or by calling 206-667-4700.

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