Research Technician I-II

Job ID
22761
Type
Regular Full-Time
Company
Fred Hutchinson Cancer Research Center
Location
US-WA-Seattle
Category
Laboratory Research Sciences

Overview

Fred Hutchinson Cancer Center is an independent, nonprofit, unified adult cancer care and research center that is clinically integrated with UW Medicine, a world leader in clinical care, research and learning. The first National Cancer Institute-designated cancer center in the Pacific Northwest, Fred Hutch’s global leadership in bone marrow transplantation, HIV/AIDS prevention, immunotherapy, and COVID-19 vaccines has confirmed our reputation as one of the world’s leading cancer, infectious disease and biomedical research centers. Based in Seattle, Fred Hutch operates eight clinical care sites that provide medical oncology, infusion, radiation, proton therapy, and related services, and network affiliations with hospitals in five states. Together, our fully integrated research and clinical care teams seek to discover new cures for the world’s deadliest diseases and make life beyond cancer a reality.

 

At Fred Hutch, we believe that the innovation, collaboration, and rigor that result from diversity and inclusion are critical to our mission of eliminating cancer and related diseases. We seek employees who bring different and innovative ways of seeing the world and solving problems. Fred Hutch is in pursuit of becoming an antiracist organization. We are committed to ensuring that all candidates hired share our commitment to diversity, antiracism, and inclusion.   



A highly collaborative laboratory-based research group in the Clinical Research Division is recruiting an outstanding Research Technician I or II to work as part of a team investigating new treatments for patients with acute myeloid leukemia (AML) primarily focused on improving existing, and developing novel, antibody-based immunotherapies, and rendering them safer for clinical application. Current projects include preclinical drug development/testing (in part with academic and industry partners), studies on mechanisms of action and resistance of antibody-based therapeutics and identification of strategies to overcome these limitations, and development of approaches to protect normal tissues from the toxicities of AML-targeted therapies (e.g. via use of genome editing).

Responsibilities

The newly hired research technician will work under the direction of the Principal Investigator and senior laboratory personnel and will be involved in a variety of techniques/duties.

 

The main focus of this position will be the following:

  • T-cell mediated cytotoxicity assays with bispecific antibodies targeting cancer cells
  • NK-cell mediated cytotoxicity assays with bispecific and trispecific antibodies and combinations of bispecific antibodies targeting cancer cells
  • Novel drug combinations of the above with clinically-approved small molecules
  • Flow cytometry-based immunophenotyping of antibodies on CRISPR-Cas9 engineered antigen-positive/-negative cell pairs

 

Additional responsibilities may include:

  • Generation and purification of recombinant antibodies and other protein therapeutics via transient and permanent expression systems
  • Conduct of immunization strategies to raise new antibodies
  • Generation of engineered/gene-edited human leukemia cells
  • Using CRISPR/Cas9 libraries for gene discovery and gene of interest/hit list follow-up
  • PBMC isolation from whole blood samples
  • Tissue culture of mammalian cell lines and patient-derived primary human cells
  • Antibody drug conjugate (ADC) cytotoxicity, antibody-dependent cellular cytotoxicity
  • FACS isolation of mammalian cells
  • Purification, cloning, and manipulation of RNA, cDNA and genomic DNA
  • Standard DNA cloning methodologies utilizing T4 DNA ligase and Gibson Assembly
  • PCR and RT-PCR, including quantitative assays using qPCR
  • Lentiviral vector modification, lentiviral particle preps and viral transduction
  • Protein gels, immunoprecipitation, western blotting, immunofluorescence microscopy
  • ELISA, antibody biotinylation, fluorescein conjugation

 

In addition:

  • Opportunities (if interested) to learn procedures with mice, in vivo imaging, and/or radioisotope handling to complement our radioimmunotherapy program
  • To be successful in this position the applicant must be: organized, independent, open to learning, and provide clear and concise communication. Comprehensive laboratory record keeping is mandatory, experience with statistical analysis and data presentation is desirable.

Qualifications

  • Bachelor’s degree in a field of biology such as immunology, cell and molecular biology, genetics, biochemistry, pathology, bioinformatics or genome sciences
  • Research Technician I:  Some laboratory experience outside of coursework.
  • Research Technician II:  2+ years previous experience working in a laboratory.
  • Must have scientific knowledge in the area of study and basic laboratory skills. 

 

Applicants must submit:

  • Cover letter discussing your interest in this position
  • Curriculum Vitae
  • Contact information for three references

 

Fred Hutch has a mandatory COVID-19 vaccine requirement, with exceptions only for approved medical or religious accommodations.
As a condition of employment, newly hired employees must provide proof of vaccination or initiate the accommodations process before their first day of employment.

 

A statement describing your commitment and contributions toward greater diversity, equity, inclusion, and antiracism in your career or that will be made through your work at Fred Hutch is requested of all finalists.

Our Commitment to Diversity

We are proud to be an Equal Employment Opportunity (EEO) and Vietnam Era Veterans Readjustment Assistance Act (VEVRAA) Employer. We are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability (physical or mental), marital or veteran status, genetic information, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to our Employee Services Center at hrops@fredhutch.org or by calling 206-667-4700.

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