• Research Technician I

    Job ID
    14874
    Type
    Regular Full-Time
    Company
    Fred Hutchinson Cancer Research Center
    Location
    US-WA-Seattle
    Category
    Laboratory Research Sciences
  • Overview

    Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutch’s pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nation’s first cancer prevention research program, as well as the clinical coordinating center of the Women’s Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.


    This research opportunity is with the McElrath Lab within the Vaccine & Infectious Disease Division. The technician will work under the direction of a Staff Scientist and use discretion and judgment in performance of duties in support of human mucosal immunology studies relevant to HIV-1 prevention. In particular, they will perform and assist in the development of mucosal explant models for evaluating the functionality, levels and distribution of HIV-1 specific antibodies. They will also be responsible for processing various mucosal specimens from study participants, performing downstream assays, collecting and analyzing the data.


    The incumbent will work closely with other laboratory staff conducting mucosal studies. They will interpret and report on experimental results and assist with data presentation for conferences and manuscripts. They may work with the clinical staff from the Fred Hutch HIV vaccine Trials Unit, University of Washington Medical Center (UWMC) and other hospitals to coordinate specimen transfer and/or personally pick up specimens. They will assist in the development and maintenance of laboratory quality control (QC) methods and records, which may require adherence to GCLP (Good Clinical Lab Practice) standards

     

    ***A cover letter is required for consideration for this position***

    Responsibilities

    • Coordinate specimen collection and transport from UWMC and other clinics
    • Coordinate sample shipping/receiving with collaborating laboratories
    • Process cervicovaginal, rectal and other mucosal tissue specimens
    • Process cervicovaginal secretions, rectal secretion and seminal plasma
    • Be available to process additional mucosal specimens as needed (e.g. cervical cytobrushes)
    • Perform ex vivo infectivity assays on mucosal tissues
    • Perform luminescence reporter assays to measure HIV infection
    • Prepare single cell suspension from mucosal specimens, such as cytobrushes and mucosal tissues
    • May prepare and cryopreserve PBMC
    • Perform microdissection of mucosal tissue to separate epithelium from underlying stroma
    • Perform and assist in developing immunohistochemistry assays on mucosal tissues
    • May perform confocal microscopy, including receptor and virus co-localization in mucosal tissue
    • Perform and assist in developing flow cytometric assays (e.g. both phenotyping and functional assays)
    • Perform and assist in developing ELISAs and related assays to measure mucosal analytes (e.g. mucosal antibodies)
    • Perform and/or help optimize new assays and specimen processing methods as needed
    • May perform and help optimize qPCR and/or digital droplet PCR assay (e.g. for detecting HIV-1 infection)
    • May prepare stocks of HIV isolates by transient transfection
    • Maintain records for experiments and quality control for the laboratory
    • Operate and carry out proper maintenance and QC of laboratory equipment
    • Prepare and maintain reagents, stocks, and cultures necessary to perform work
    • Order and maintain laboratory supplies
    • Other duties as assigned

    Qualifications

    A bachelor’s degree in a biological science and some bench research experience or the equivalent combination of education and experience. Experience with cellular immunologic assays, tissue culture, micro-dissection, molecular biology and microscopy is desirable but not essential. Motivation and adaptability are essential, as is a keen interest in the HIV vaccine and prevention fields. Must be willing to work with biohazardous/infectious agents (e.g., blood-borne pathogens, including HIV-1), including human tissues under carefully controlled conditions. Strong organizational and communication skills are necessary. Must be flexible and willing to work some evenings and weekends.

    Our Commitment to Diversity

    We are proud to be an Equal Employment Opportunity (EEO) and Vietnam Era Veterans Readjustment Assistance Act (VEVRAA) Employer. We are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability (physical or mental), marital or veteran status, genetic information, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to our Employee Services Center at escmail@fredhutch.org or by calling 206-667-4700.

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