• Research Technician I - Microbiology/Bacterial Transformation

    Job ID
    Regular Full-Time
    Fred Hutchinson Cancer Research Center
    Laboratory Research Sciences
  • Overview

    Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutch’s pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nation’s first cancer prevention research program, as well as the clinical coordinating center of the Women’s Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.


    The Johnston Laboratory is an interdisciplinary and highly collaborative team of classically trained microbiologists, synthetic microbiologists, bioinformaticians and engineers. Using cutting edge technologies and methodologies, we seek to gain a deeper functional understanding of what bacteria are doing and how they are doing it, and then to use that information to create novel therapeutics and engineer the next generation of microbe-based technologies for application in human medicine, synthetic microbiology and bioengineering.


    The Johnston Laboratory in the Vaccine and Infectious Disease Division at Fred Hutch is recruiting a highly motivated and resourceful research technician with expertise in the genetic engineering/transformation of non-model bacterial species and general microbiology to join our cutting-edge microbial genomics efforts.


    The position is intended to provide support for our ongoing microbial engineering projects and microbial technology developments, as well as supporting general laboratory responsibilities. The successful candidate will work with the PI and post-doctoral fellows using state-of-the-art approaches in synthetic microbiology and genomics in a highly collaborative, stimulating, and collegial research environment. The candidate will also assist in lab maintenance, including responsibility of ordering lab reagents/supplies and making of communal lab reagents. With interest and experience in our lab, the individual may assist with additional opportunities in research. This position will provide ample opportunities for professional development and the acquisition of cutting-edge skills in synthetic microbiology research, research planning and strategy, and data analysis and interpretation. The Johnston lab especially encourages those from backgrounds poorly represented in STEM fields to apply to the lab. Specific duties may include:

    • Aerobic and anaerobic bacterial culture and stock maintenance, genetic engineering/transformation of the such strains.
    • Adhere to standard operating procedures (SOPs) for isolation and analysis of DNA and RNA from microbial specimens, including PCR, quantitative PCR, RT-PCR, and sequencing.
    • Maintenance of general lab supplies, consumables and equipment
    • Conduct diligent record-keeping and reporting
    • Ordering/cataloging of laboratories supplies and reagents
    • Presenting findings at internal (Johnston Lab) meetings
    • Other research or organizational duties, as appropriate


    Please include a cover letter with your application, detailing your interest and suitability for this position.


    Minimum qualifications:

    • Bachelor's degree with 1-2 years’ experience in microbial research setting
    • Highly-motivated individual interested in a career in biological research or medicine, with a keen interest in genetic engineering of microbes
    • Technically meticulous when conducting experiments
    • Highly organized in record-keeping
    • Independent, intellectually passionate and able to display scientific initiative
    • Excellent teamwork, time management and organizational skills
    • Ability to work independently in a dynamic and results-oriented environment
    • Ability to meet deadlines and multitask efficiently is a must


    Preferred qualifications:

    • Basic molecular biology and microbiology techniques, such as aseptic technique, bacterial isolation and culture, strain library maintenance, PCR, DNA/RNA/protein extraction, cloning, recombineering, etc. are stringently required.
    • A working knowledge and previous wet-lab experience with transformation of non-model bacterial organisms (optimization of competent cell preparation/heat shock/electroporation), in addition to standard coli transformations, is highly desirable and preferred.
    • Experience in the design and use of reagents for DNA cloning and genome engineering with shuttle vectors, suicide-vectors, CRISPR/Cas9 and transposon mutagenesis technologies also desirable.
    • Working knowledge and experience in anaerobic culture (anaerobic chamber use) will be beneficial to the role.

    Our Commitment to Diversity

    We are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We are proud to be an Equal Opportunity and VEVRAA Employer. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability, marital or veteran status, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to our Employee Services Center at escmail@fredhutch.org or by calling 206-667-4700.


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