• Research Technician I-II - X-Ray Crystallography

    Job ID
    14542
    Type
    Regular Full-Time
    Company
    Fred Hutchinson Cancer Research Center
    Location
    US-WA-Seattle
    Category
    Laboratory Research Sciences
  • Overview

    Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutch’s pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nation’s first cancer prevention research program, as well as the clinical coordinating center of the Women’s Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.


    The Stamatatos/Pancera lab, within the Vaccine and Infectious Disease Division, works towards the development of safe and effective vaccines against HIV-1 and malaria. Our major emphasis is the design of protein immunogens and understanding the immune response following vaccination or natural infection. The subgroup led by Dr. Pancera focuses on structural characterization of these immunogens and their immune responses. The laboratory utilizes a wide range of in vitro and in vivo systems and cutting-edge technologies and techniques. Experimental approaches include high-throughput crystallography, specialized immunological methodologies and assays, molecular and cellular biology techniques, bioinformatics, and computational approaches.


    Our lab is seeking an outstanding Research Technician I-II to work on a project involving the characterization of the humoral immune response following vaccination against HIV, malaria or other pathogens using X-ray crystallography and cryoEM and the designs of novel immunogens.

    Responsibilities

    1. Responsible for crystallization of the proteins/antibodies complexes and for solving and refining their structures
    2. Conduct protein expression and purification experiments, including affinity purification, Ion exchange and FPLC, related to the production of antibodies against viral glycoproteins for structural biology
    3. May involve use of Electron Microscopy, molecular modelling or structure-based mutagenesis and design
    4. May involve use of Bio Layer interferometry, SPR and ELISA
    5. Initiate and carry out scientific projects, under the supervision of Dr. Pancera
    6. Develop and demonstrate a thorough understanding of the scientific literature relating to the research topics.

    Qualifications

    The ideal candidate must be a ‘risk taker’, show initiative, willingness to take up new skills and responsibilities, and be a team player. Excellent attention to details and the ability to work independently are essential to success in this position, as are good communication and organizational skills. The candidate must be self-motivated, able to multitask and to work within a very collaborative environment. Candidates with backgrounds in areas such as virology, protein biochemistry, vaccine development are strongly encouraged to apply.

     

    Required:

    • BS with 1-2 years of experience or MS is required
    • Significant expertise in X-ray protein crystallography highly preferred
    • On-hands experience with diverse aspects of Molecular Biology

    Preferred:

    • Knowledge of Cryo-EM
    • Experience in X-ray crystallography including up-to-date knowledge of relevant programs including CCP4, and Phenix packages, protein purification, protein characterization, and a strong publication history
    • Expertise with tissue culture and immunological assays

    Our Commitment to Diversity

    We are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We are proud to be an Equal Opportunity and VEVRAA Employer. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability, marital or veteran status, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to our Employee Services Center at escmail@fredhutch.org or by calling 206-667-4700.

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