• Research Technician II-III: Cell culture - gene editing & process development

    Job ID
    Regular Full-Time
    Fred Hutchinson Cancer Research Center
    Laboratory Research Sciences
  • Overview

    Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutch’s pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer with minimal side effects. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nation’s first and largest cancer prevention research program, as well as the clinical coordinating center of the Women’s Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.


    As a Research Tech Level II, the position acts both independently and as a team collaborator within the lab to lead multiple laboratory projects including but not limited to isolation, enrichment, transduction and expansion of T cells/Stem Cells, complex analytical procedures, data compilation/interpretation, and generating detailed technical reports/presentations. In addition, this position helps identify emerging technologies and automation, monitors industry trends and conducts evaluations for feasibility. This position works with and under the guidance of a lab supervisor for experimental design, study execution, data analysis and data interpretation. The candidate also interacts heavily with internal and external research and principal investigator labs, GMP manufacturing, Quality Control, Quality Assurance, and other scientific/administrative staff, as needed.


    The position focuses on the development, optimization, and transfer of cellular and gene therapy unit operations including cell selections from starting materials, genetic modifications (viral transduction, electroporation, etc.), growth/expansion conditions, media/cytokine formulations, cell harvests, cryopreservation, and freeze-thaw recovery. In addition, this position collaborates to develop robust and repeatable analytical methods to characterize starting, in-process and final, formulated cellular products. Ultimately, development activities generate robust, repeatable and scalable processes to support toxicology studies, technology transfer/product introduction into the Fred Hutch GMP Cell Processing manufacturing facility, and IND generation to enable clinical trial initiation. 


    Required Qualifications:

    • Research Tech II: BS/MS in a biological sciences discipline, and 2+ years' (BS) or 1+ years' (MS) experience in mammalian cell culture
    • Research Tech III: BS/MS in a biological sciences discipline, and 5+ years' experience in mammalian cell culture or Process Development
    • Experience in one or more of the following technical areas:
      • Adherent and/or suspension cell cultures in flasks and/or bioreactors (fed-batch, perfusion, disposable bioreactor systems, etc.).
      • Cell culture media development and optimization.
      • Associated upstream and downstream unit operations such as cell banking, virus removal, purification, etc.
      • Cryopreservation and recovery of cryopreserved cells.
      • Experience with gene editing platforms (i.e. Crispr/cas9 system)

    Preferred Experience:

    • Demonstrated experience trouble-shooting issues associated with process scale-up, transfer into a GMP manufacturing setting, and generating process transfer documentation.
    • Direct experience in a Process Development environment supporting aseptic cell culture-based manufacturing operations
    • 1+ years hands-on experience with flow cytometry and T-cell/stem cell isolation/expansion protocols

    Our Commitment to Diversity

    We are proud to be an Equal Employment Opportunity (EEO) and Vietnam Era Veterans Readjustment Assistance Act (VEVRAA) Employer. We are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability (physical or mental), marital or veteran status, genetic information, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to our Employee Services Center at escmail@fredhutch.org or by calling 206-667-4700.


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