• Research Technician II/III, Pancreas Cancer Research

    Job ID
    14397
    Type
    Regular Full-Time
    Company
    Fred Hutchinson Cancer Research Center
    Location
    US-WA-Seattle
    Category
    Laboratory Research Sciences
  • Overview

    Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutch’s pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nation’s first cancer prevention research program, as well as the clinical coordinating center of the Women’s Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.


    The Hingorani Laboratory investigates molecular and cellular mechanisms of pancreatic ductal adenocarcinoma (PDA) pathogenesis. The murine models of distinct genetic subtypes of pancreas cancer that we have developed form the basis of our integrated, systematic, and multidisciplinary research program. Our efforts are directed at identifying and overcoming stromal barriers to treatment (including elevated interstitial pressures, activated fibroblasts, and multiple mechanisms of immune suppression); identifying and disrupting molecular drivers of metastasis; and developing immune-based treatment strategies. Our ultimate aim is to take our discoveries and reduce them to practice in the clinic in order to radically transform the approach and prognosis for patients with this devastating disease.


    This Research Technician II/III position represents an excellent opportunity for an experienced, highly motivated individual with a passion for science to work and learn in a dynamic scientific environment while addressing important problems relevant for the successful treatment of cancer.

    Responsibilities

    The research technician will be involved in all aspects of murine preclinical studies including but not limited to breeding, genotyping, ultrasound and other imaging techniques, drug treatments, and performing necropsies to obtain tissues for histology and cell lines. The technician may be involved in derivation, maintenance and study of primary cell lines; isolation of DNA, RNA, and proteins; as well as other techniques such as FACS sorting and expanding cells in vitro, analyzing cell phenotype and function using techniques including flow cytometry, proliferation assays, cytotoxicity assays, PCR, RT-PCR, sequencing, and ELISpot. Molecular techniques used in the lab include cloning, transfection/transduction of cell lines, gene targeting and northern and Southern blotting. Biochemical and cellular techniques include SDS-PAGE, western blotting, immunofluorescence, and immunohistochemistry. The technician will also assist with the management and organization of a fast-paced lab, and perform other duties as assigned by supervisor

    Qualifications

    • A bachelor's degree in bioengineering, biophysics or biological sciences and at least 5 years of laboratory research experience including small animal surgeries and injections, cell tissue culture, flow cytometry, and molecular biology techniques are required.
    • A master's degree in a biological field and at least 2 years of experience in the techniques listed above are highly desired.
    • Computer skills: Microsoft Excel, Prism (Graphpad), FlowJo, and statistical programs for data analysis
    • Excellent organizational and interpersonal skills are required.
    • Strong troubleshooting skills and the ability to multi-task with precise attention to detail is especially critical.
    • Experience with genetically engineered murine models of pancreas cancer or other solid tumors is desired.

    Our Commitment to Diversity

    We are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We are proud to be an Equal Opportunity and VEVRAA Employer. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability, marital or veteran status, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to our Employee Services Center at escmail@fredhutch.org or by calling 206-667-4700.

    Options

    Sorry the Share function is not working properly at this moment. Please refresh the page and try again later.
    Share on your newsfeed