Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutch’s pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nation’s first cancer prevention research program, as well as the clinical coordinating center of the Women’s Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.
This senior level technician will work independently under the direction of the principal investigator and scientific staff to design experiments; conduct literature reviews to assist in determining most suitable methods to be used in research and examine proposals to determine projects and methodologies best suited to research objectives and the overall goals of the laboratory. The incumbent will summarize and write for publication or presentation the results of research projects.
The research technician will be involved in all aspects of murine preclinical studies in the Hingorani Laboratory, including but not limited to breeding, genotyping, ultrasound and other imaging techniques, and performing necropsies to obtain tissues for histology and cell lines. The technician may be involved in derivation, maintenance and study of primary cell lines; isolation of DNA, RNA, and proteins; as well as other techniques such as FACS sorting and expanding cells in vitro, analyzing cell phenotype and function using techniques including flow cytometry, proliferation assays, cytotoxicity assays, PCR, RT-PCR, sequencing, and ELISpot. Molecular techniques used in the lab include cloning, transfection/transduction of cell lines, gene targeting and northern and Southern blotting. Biochemical and cellular techniques include SDS-PAGE, western blotting, immunofluorescence, and immunohistochemistry. The technician will also assist with the management and organization of a fast-paced lab, and perform other duties as assigned by supervisor.